CRISPR/Cas9, Genome Editing Tool

What is CRISPR?

CRISPR/Cas9, is an RNA-guided targeted genome editing tool – allows researchers to do gene knockout, knockin SNPs, insertions and deletions in cell lines and animals. The CRISPR/Cas9 genome editing system requires two components, Cas9, the endonuclease, and a guide RNA (sgRNA); sgRNA guides Cas9 to the location in the genome sequence specifically. With the protospacer-adjacent motif (PAM - the sequence NGG) present at the 3′ end, Cas9 will unwind the DNA duplex and cleave both strands upon recognition of a target sequence by the guide RNA.
Learn more from CRISPR videos.

CRISPR Products and Services

OriGene provides reagents to support a broad range of CRISPR/Cas9 genome editing applications. Click on any genome editing related product below and learn more about how we can help you.

View the original nature review article

CRISPR Resources