OriGene Technologies, Inc.
  邮箱:  密码: 忘记密码?
注册 | 购物车 | 主页
搜索:    
About FL cDNA Clones RNAi Gene Expression Lysates and Proteins Antibodies Tissue IVD 原料 Other Products Tech Support
orange
 
Home CRISPR/Cas9 AAVS1/ROSA26 Transgene Insertion

AAVS1/ROSA26 Transgene Insertion

divider

Genome-editing
Knockout Kit
CRISPR/Cas9
Vectors
gRNA Cloning and
Donor Construction
AAVS1/ROSA26
Transgene Insertion

CRISPR/Cas
Starter Kit

Targeted Transgene Insertion into the AAVS1 Locus

Adeno-associated virus integration site 1 (AAVS1) in human genome is a safe harbor for transgene integration. It is transcriptionally active and transgene expression from this site is robust and stable. CRISPR technology is ideal for targeted transgene insertion at the AAVS1 locus.

AAVS1 Safe Harbor knockin system via CRISPR

AAVS1_simple_diagram

This system needs two components:

  1. pCas-Guide-AAVS1, All-in-one CRISPR vector for AAVS1 targeting
  2. pAAVS1-Puro-DNR, donor vector for transgene integration, containing the AAVS1 homologous arms (Gene of interest needs to be cloned)


AAVS1 gRNA Vector

Cat # Product Name Description Price Availability Manual
GE100023 pCas-Guide-AAVS1 AAVS1 gRNA vector, validated AAVS1 targeting sequence cloned in pCas-Guide vector ¥3420 2-3周 Manual

AAVS1 Donor Vector

Cat # Product Name Description Price Availability Manual
GE100024 pAAVS1-Puro-DNR Donor vector with AAVS1 homologous arms cloned, ready for transgene cloning ¥5510 2-3周 Manual
GE100035 pAAVS1-BSD-DNR donor vector with AAVS1 homologous arms cloned (Blasticidin selection), ready for exogenous gene cloning (10 ug) ¥5510 2-3周 Manual

Transgene Knockin Vector Kits

Cat # Product Name Description Price Availability Manual
GE100027 AAVS1 Transgene knockin vector kit Vector kit for targeted transgene insertion into AAVS1 locus, including GE100023, GE100024 and GE100003 ¥8360 2-3周 Manual
GE100036 AAVS1 transgene knockin kit (BSD) Vector kit for targeted transgene insertion into AAVS1 locus (blasticidin selection), including GE100023, GE100035 and GE100003 ¥8360 2-3周 Manual

ROSA26 gRNA Vector

Cat # Product Name Description Price Availability Manual
GE100050 pCas-Guide-ROSA26 ROSA26 safe harbor gRNA vector, validated ROSA26 gRNA targeting sequence cloned in pCas-Guide vector ¥4275 现货 Manual
GE100051 pROSA26-Puro-DNR ROSA26 donor vector with ROSA26 homologous arms cloned (Puro selection), ready for exogenous gene cloning (10 ug) ¥7125 现货 Manual
GE100052 ROSA26 CRISPR transgene knockin kit (Puro) Vector kit for CRISPR targeted transgene insertion into ROSA26 locus in mice (Puro), including GE100050, GE100051 and GE100003 ¥9975 现货 Manual

Scheme of AAVS1/ROSA26 transgene knockin via CRISPR

AAVS1_simple_diagram AAVS1_simple_diagram

Related Products

Cas9 insertion at AAVS1 locus RFP AAVS1 donors
(AAVS1 positive control)
AAVS1-int5 PCR Primer Pairs


 

bar
全国免费电话:400-6744-363(手机),800-9904-363(座机)订货邮箱:orderscn@origene.com,techsupport@origene.cn
电话:+86-10-59755312 传真:+86-10-59755322-888 地址:北京市经济技术开发区科创14街99号汇龙森科技园11栋
京ICP证07031721号 Copyright © 2019 北京傲锐东源(OriGene Technologies)生物科技有限公司,All Rights Reserved. 法律声明.