UltraMAB? Antibodies - Validated with 10K Protein Chip
Western blot of whole cell lysate (25ug) (1: MCF7; 2: HeLa; 3: Jurkat; 4: PC-12; 5: COS-7; 6: A549). The labelled upper band is Pro-Met and the labelled lower band is Met. Dilution: 1:500
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# <a href="/other/products/LC810HELA.aspx">LC810HELA</a>) and MET-Knockout hela cells (KO,Cat# <a href="/KO_Lysate/MET/LC810144.aspx">LC810144</a>) were separated by SDS-PAGE and immunoblotted with anti-MET monoclonal antibody <a href="/Antibody/UM800082.aspx">UM800082</a>. Then the blotted membrane was stripped and reprobed with anti-ß-actin (<a href="/Antibody/TA811000.aspx">TA811000</a>) as a loading control.(1:500)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MET mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110C for 10 min, UM800082)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MET mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110C for 10 min, UM800082)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MET mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110C for 10 min, UM800082)(1:150)
Immunohistochemical staining of paraffin-embedded human renal carcinoma using anti-MET clone UMAB190 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM800082 requires heat-induced epitope retrieval with ACCEL in a presure cooker for 3 minutes at 110C. The image shows strong membranous and cytoplasmic staining in the tumor cells of the kidney.
Immunohistochemical staining of paraffin-embedded human adjacent normal lung using anti-MET clone UMAB190 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM800082 requires heat-induced epitope retrieval with ACCEL in a presure cooker for 3 minutes at 110C. The image shows strong membranous and cytoplasmic in brochial tubes.
Immunohistochemical staining of paraffin-embedded human melanoma using anti-MET clone UMAB190 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM800082 requires heat-induced epitope retrieval with ACCEL in a presure cooker for 3 minutes at 110C. The image shows strong membranous and cytoplasmic staining in epithelial cells.
Immunohistochemical staining of paraffin-embedded human placenta using anti-MET clone UMAB190 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM800082 requires heat-induced epitope retrieval with ACCEL in a presure cooker for 3 minutes at 110C. The image shows strong membranous and cytoplasmic staining in the trophoblasts.
Immunofluorescent staining of HeLa cells using anti- MET mouse monoclonal antibody (UM800082, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq, Jul 2008)
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Western blot of whole cell lysate (25ug) (1: MCF7; 2: HeLa; 3: Jurkat; 4: PC-12; 5: COS-7; 6: A549). The labelled upper band is Pro-Met and the labelled lower band is Met. Dilution: 1:500
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and MET-Knockout hela cells (KO,Cat# LC810144) were separated by SDS-PAGE and immunoblotted with anti-MET monoclonal antibody UM800082. Then the blotted membrane was stripped and reprobed with anti-?-actin (TA811000) as a loading control.(1:500)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MET mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110C for 10 min, UM800082)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MET mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110C for 10 min, UM800082)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MET mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110C for 10 min, UM800082)(1:150)
Immunohistochemical staining of paraffin-embedded human renal carcinoma using anti-MET clone UMAB190 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM800082 requires heat-induced epitope retrieval with ACCEL in a presure cooker for 3 minutes at 110C. The image shows strong membranous and cytoplasmic staining in the tumor cells of the kidney.
Immunohistochemical staining of paraffin-embedded human adjacent normal lung using anti-MET clone UMAB190 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM800082 requires heat-induced epitope retrieval with ACCEL in a presure cooker for 3 minutes at 110C. The image shows strong membranous and cytoplasmic in brochial tubes.
Immunohistochemical staining of paraffin-embedded human melanoma using anti-MET clone UMAB190 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM800082 requires heat-induced epitope retrieval with ACCEL in a presure cooker for 3 minutes at 110C. The image shows strong membranous and cytoplasmic staining in epithelial cells.
Immunohistochemical staining of paraffin-embedded human placenta using anti-MET clone UMAB190 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM800082 requires heat-induced epitope retrieval with ACCEL in a presure cooker for 3 minutes at 110C. The image shows strong membranous and cytoplasmic staining in the trophoblasts.
Immunofluorescent staining of HeLa cells using anti- MET mouse monoclonal antibody (UM800082, green, 1:50). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue).
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