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Anti-CAS9 Antibody

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Specifications Related Products Product Reviews FAQs
Catalog No. Description Amount Price Shipping
TA190309
  • Rabbit polyclonal CAS9 Antibody
100ul 3088 1-2周 Add to Shopping Cart
TA190309S
  • Rabbit polyclonal CAS9 Antibody
30ul850现货Add to Shopping Cart
   TrueMABTM Antibodies
* Delivery is in business days
WB(1)
IP(1)
Gene Name Synonyms:
ImmunogenCAS9 Synthetic peptide conjugated to KLH derived from within residues 1150-1200 of S. Pyogenes enzyme cas9 (Used in OriGene CRISPR/Cas9 Genome Editing system )
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name Isotype
Species ReactivityS. Pyogenes Concentration1.00mg/ml
Purification Affinity purified
Guaranteed Application *WB, IP Suggested DilutionsWB 1:500
Background
Related Pathway

* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T were transfected with either WT CAS9 plasmid (GE100002), CAS9 nickase plasmid (GE100019) or control vector (PS100001). Equivalent amount of each cell lysates (10ug per lane) were immunoblotted with TA190309 (1:2000).
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IP Image
Immunoprecipitation of WT Cas9 and Cas9 nickase by using TA190309. HEK293T were transfected with either WT CAS9 plasmid (GE100002), CAS9 nickase plasmid (GE100019), or negative control vector (PS100001). Input: 10ug total cell lysates; WB was performed using TA190309 (1:2000). IP: 100ug of cell lysates were precipitated with 20ug of TA190309 or control antibody, and 5ug Protein G Beads. Mouse anti-DDK monoclonal antibody (TA50011) was used in WB to detect the immuno-precipitated Cas9 (as Cas9 is Myc-DDK tagged).
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