Purified from mouse ascites fluids by affinity chromatography
Guaranteed Application *
WB, IHC
Suggested Dilutions
WB 1:2000, IHC 1:150,
Background
This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012].
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYMP (RC202170, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYMP.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and TYMP-Knockout HeLa cells (KO, Cat# LC831279) were separated by SDS-PAGE and immunoblotted with anti-TYMP monoclonal antibody TA801109(1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TYMP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801109)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TYMP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801109)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TYMP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801109)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TYMP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801109)
Researchers who bought this antibody have also purchased:
