beta Catenin (CTNNB1) (core) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9G10]

Specifications

Product Data
Clone Name	9G10
Applications	ELISA, IF, IHC, IP, WB
Recommend Dilution	Western Blot: 0.5 μg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.  Included Positive Control: Cell Lysate from Untreated SW480 cells. ELISA: 0.05 μg/ml. Immunoprecipitation: 1-10 μg per 106 vanadate treated A431 cells. Immunocytochemistry: 0.1-1 μg/ml.  Immunohistochemistry on Paraffin Sections.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant β-Catenin.  Epitope: Core (armadillo repeats).
Specificity	This antibody specifically interacts with the core region (armadillo repeats) of β-Catenin.
Formulation	1 ml 2 x PBS State: Purified State: Lyophilized purified Ig fraction from Serum-Free Cell Culture Supernatant Stabilizer: PEG and Sucrose Preservative: 0.09% Sodium Azide
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subesequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Predicted Protein Size	90 kDa
Gene Name	catenin beta 1
Database Link	Entrez Gene 1499 Human UniProt ID P35222
Background	The α-, β- and γ-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The direct interaction of β-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighbouring cells. Recent studies indicate that β-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. β-catenin directly interacts and constitutively activates transcription factors of the TCF/LEF gene family. Thus it is proposed that β-catenin plays a dual role not only in the maintainance and regulation of cell-cell interactions but also in the regulation of gene activity.
Synonyms	CTNNB1, CTNNB, Beta-catenin
Note	Protocol: Included Positive Control: SW480 Untreated Formulation: Lyophilized Cell Lysate from Serum starved SW480 Cells. Stability: Restore by addition of 200 μl H2O. After complete solubilization add 200 μl 2x SDSPAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The Positive Control cell lysate is recommended for immunoblot applications. 20 μl of positive control cell lysate corresponds to ca 20.000 cells. Use 20 μl/lane (mini gel) for HRPO/ECL detection of the target proteins. Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as Immunoprecipitation. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Shelf life: one year from despatch.
Reference Data

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