beta Catenin (CTNNB1) (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7D8]

Specifications

Product Data
Clone Name	7D8
Applications	ELISA, IF, IHC, IP, WB
Recommend Dilution	ELISA: 0.05 μg/ml. Western Blot: 0.5 μg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from untreated SW480 cells. Immunoprecipitation: 1-10 μg per 10e6 vanadate treated A431 cells. Immunocytochemistry: 0.1-1 μg/ml. Immunohistochemistry on Paraffin Sections.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant beta-Catenin
Specificity	This antibody specifically interacts with the C-terminus (exon 14) of beta-Catenin.
Formulation	1ml 2xPBS containing 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction
Purification	Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	catenin beta 1
Database Link	Entrez Gene 1499 Human UniProt ID P35222
Background	The α-, β- and γ-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The direct interaction of β-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighbouring cells. Recent studies indicate that β-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. β-catenin directly interacts and constitutively activates transcription factors of the TCF/LEF gene family. Thus it is proposed that β-catenin plays a dual role not only in the maintainance and regulation of cell-cell interactions but also in the regulation of gene activity.
Synonyms	CTNNB1, CTNNB, Beta-catenin
Note	Protocol: Positive Control: Cell lysate from untreated SW 480 cells. Format: Lyophilized cell lysate from Serum starved SW 480 cells Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.