EGFR pSer1047 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1H9]

CAT#: AM00036PU-N

EGFR pSer1047 (incl. pos. control) mouse monoclonal antibody, clone 1H9, Purified

Conjugation: Unconjugated Biotin



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CNY 5,973.00


货期*
5周

规格
    • 100 ug

Cited in 5 publications.

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Specifications

Product Data
Clone Name 1H9
Applications ELISA, IF, IP, WB
Recommend Dilution Western blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.1 µg/ml.
Immunoprecipitation: 1-10 µg per 10e6 pervanadate-treated A431 cells.
Immunocytochemistry: 0.1-1 µg/ml.
Luminex.
Included Positive Control: Cell lysate from pervanadate-treated HepG2 cells (See Protocols below).
Reactivity Canine, Human, Mouse
Host Mouse
Clonality Monoclonal
Immunogen Phosphopeptide conjugated to KLH. 
Epitope: Phosphoserine 1047, RYSpSDST
Specificity This antibody recognizes EGFR phosphorylated at Serine 1047.
Formulation PBS containing 0.09% Sodium Azide, PEG and Sucrose
State: Purified
State: Lyophilized purified Ig fraction
Reconstitution Method Restore with 1 ml H2O (15 min, RT).
Purification Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation Unconjugated
Storage Condition Store lyophilised product upon arrival at -20°C.
Following reconstitution aliquot and store at 2-8°C for up to three months or freeze in liquid nitrogen at -80°C for longer.
Avoid repeated freezing and thawing.
Should this product contain a precipitate, we recommend centrifugation before use.
Predicted Protein Size 180 kDa
Gene Name epidermal growth factor receptor
Background EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y845 by src family kinases. Phosphorylation of Y1045 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1047 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T654 (by PKC) and T669 (by MAPK, p38) interferes with receptor endocytosis/recycling.
Synonyms Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Note Protocol: Included Positive Control: Cell lysate from pervanadate-treated HepG2 cells.

Format: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15 min with pervanadate.

Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation.

Storage: Aliquote reconstituted product and store frozen. Avoid repeated freezing and thawing.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (5)

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