EGFR pTyr1173 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9H2]

Specifications

Product Data
Clone Name	9H2
Applications	ELISA, IF, IHC, IP, WB
Recommend Dilution	Western Blot: 0.5 μg/ml for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blot incubation buffer CPPT (AS00002BU-L/-N). Included Positive Control: Cell lysate from vanadate-treated HepG2 cells. Immunoprecipitation: 1-10 μg per 10e6 vanadate treated A431 cells. Immunocytochemistry: 1-10 μg/ml. Immunohistochemistry. ELISA: 0.05 µg/ml.
Reactivity	Canine, Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Phosphopeptide conjugated to KLH.  Epitope: phospho-Tyr1173 N A E pY L R V
Specificity	The antibody specifically interacts with the 1197 - N A E pY L R V motif corresponding to the major autophosphorylation site of Human EGFR. Mab 9H2 does not crossreact with the highly homologous pTyr1248 of acticated erbB2.
Formulation	PBS with 0.09% Sodium Azide, PEG and Sucrose Label: Biotin State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Subsequent thiophilic adsorption and size exclusion chromatography
Conjugation	Biotin
Storage Condition	Store the antibody (aliquote in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	epidermal growth factor receptor
Database Link	Entrez Gene 1956 Human UniProt ID P00533
Background	EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.
Synonyms	Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Note	Protocol: Provided Positive Control cell lysate: HepG2 Pervanadate Treated -recommended for Immunoblotting Formulation: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15 min. with pervanadate. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Store in aliquots at -20°C. Avoid repeated freezing and thawing. Application: 20 µl of positive cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysate contains SDS and is not recommended for applications with native proteins such as Immunoprecipitation !
Reference Data

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