Insulin Receptor (INSR) pTyr1322 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 21G12]

Specifications

Product Data
Clone Name	21G12
Applications	ELISA, WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from pervanadate-treated HEK-293 cells. ELISA: 0.1 µg/ml.
Reactivity	Canine, Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjugated to KLH.  Epitope: Phosphotyrosine 1322 (HIPpYTHM)
Specificity	This antibody specifically recognizes Insulin receptor phosphorylated at Tyrosine 1322.
Formulation	1 ml 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	insulin receptor
Database Link	Entrez Gene 3643 Human UniProt ID P06213
Background	The insulin receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. The insulin receptor is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional phosphorylation sites such as tyrosine residues 960, 1316, and 1322 regulate the assembly of signal transduction complexes.
Synonyms	Insulin Receptor
Note	Protocol:  Positive Control: Cell lysate from pervanadate-treated HEK-293 cells.  Format: Lyophilized cell lysate from HEK-293 cells.  Serum starved cells were treated for 15 minutes with pervanadate. Reconstitution: Restore by addition of 200 μl H2O. After complete solubilization add 200 μl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 μl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 μl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation.
Reference Data

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