AKT1 non pSer473 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11A11]

Specifications

Product Data
Clone Name	11A11
Applications	ELISA, WB
Recommend Dilution	Suitable for Western blot: 0.5 µg/ml for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Use cell lysate from untreated HepG2 cells (provided with this antibody). ELISA: 0.05 µg/ml.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to KLH. Epitope: C-terminus.
Specificity	11A11 specifically recognizes PKB when it is dephosphorylated at serine 473.
Formulation	State: Purified State: Lyophilized from 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose.
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent thiophilic adsorption and size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freeze / thaw cycles.
Gene Name	AKT serine/threonine kinase 1
Database Link	Entrez Gene 207 Human UniProt ID P31749
Background	The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far; the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulation of cells with growth factors leads to activation of PKB by a phosphoinositide-3-kinase dependent signal transduction pathway.
Synonyms	Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT
Note	A positive control cell lysate is provided - please see "Protocols" for details. Protocol: POSITIVE CONTROL CELL LYSATE: HepG2 untreated Format: Lyophilized cell lysate from serum starved HepG2 cells Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Aliquote and store frozen, avoid repeated freezing and thawing. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. PLEASE NOTE: The lyophilized cell lysates contain SDS and are NOT recommended for applications with native proteins such as immunoprecipitation.
Reference Data

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