AKT1 (specific) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5G12]

Specifications

Product Data
Clone Name	5G12
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: Use at 0.05 µg/ml.  Western Blot: Use at 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from untreated Neuro2A cells. The antiboby crossreacts in immunoblot applications with an unknown protein of ca. 120kDa expressed in human tumor cell lines. Immunohistochemistry.
Reactivity	Canine, Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to KLH. Epitope: aa 107-122
Specificity	This antibody specifically recognizes the alpha isoform of PKB (PKBα/Akt1).
Formulation	PBS containing 0.09% Sodium Azide, PEG and Sucrose/50% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Predicted Protein Size	60 kDa
Gene Name	AKT serine/threonine kinase 1
Database Link	Entrez Gene 207 Human UniProt ID P31749
Background	The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far; the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulation of cells with growth factors leads to activation of PKB by a phosphoinositide-3-kinase dependent signal transduction pathway.
Synonyms	Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT
Note	Protocol: Positive Control Cell lysate provided Neuro 2A untreated Formulation: Lyophilized cell lysate from serum starved neuro 2A cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Store in aliquots at -20°C. Avoid repeated freezing and thawing. Applications: The Positive Control Cell Lysate is recommended for Immunoblotting. 20 µl of positive cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Note: The lyophilized cell lysate contains SDS and are not recommended for applications with native proteins such as Immunoprecipitation.
Reference Data

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