AKT1 (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5C10]

Specifications

Product Data
Clone Name	5C10
Applications	ELISA, WB
Recommend Dilution	ELISA: Use at 0.05 µg/ml. Western Blot: use at 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: HepG2 untreated (See Protocols).
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to KLH. Epitope: C-terminus (aa 466-480).
Specificity	This antibody specifically recognizes the C-terminus of protein kinase B (aa 466-480).
Formulation	1 ml 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 1 ml H2O (15 min, RT)
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Predicted Protein Size	60 kDa
Gene Name	AKT serine/threonine kinase 1
Database Link	Entrez Gene 207 Human UniProt ID P31749
Background	The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far; the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulation of cells with growth factors leads to activation of PKB by a phosphoinositide-3-kinase dependent signal transduction pathway.
Synonyms	Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT
Note	Protocol: Positive Control Cell lysate provided HepG2 unTreated Formulation: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Store in aliquots at -20°C. Avoid repeated freezing and thawing. Applications: The Positive Control Cell Lysate is recommended for Immunoblotting. 20 µl of positive cell lysate correspond to ca. 80.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Note: The lyophilized cell lysate contains SDS and are not recommended for applications with native proteins such as Immunoprecipitation.
Reference Data

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