STAT5A pTyr695/699 Mouse Monoclonal Antibody [Clone ID: 5G4]

Specifications

Product Data
Clone Name	5G4
Applications	ELISA, IF, IHC, WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 µg/ml. Immunohistochemistry. Immunocytochemistry.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjuagted to KLH
Specificity	This antibody specifically recognizes STAT5A phosphorylated at Tyr 694 and STAT5B phosphorylated at Tyr 699. The antibody does not crossreact with the non-phosphorylated form of STAT5 nor with unrelated tyrosine-phosphorylated proteins.
Formulation	PBS/0.09% Na-Azide/PEG and Sucrose/50% Glycerol State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody (Aliquote and freeze in liquid nitrogen) at -20°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	signal transducer and activator of transcription 5A
Database Link	Entrez Gene 6776 Human UniProt ID P42229
Background	The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. STAT5 is activated in response to prolactine, growth hormone, GM-CSF, and IL-2.
Synonyms	STAT-5, STAT-5A, Mammary gland factor, MGF
Reference Data

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