Phosphotyrosine Mouse Monoclonal Antibody [Clone ID: PY20]

Specifications

Product Data
Clone Name	PY20
Applications	ELISA, IF, IHC, IP, WB
Recommend Dilution	ELISA: 100 ng/ml. Immunofluorescence.  Western blotting: 2 µg/ml.  For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background. Immmunoprecipitation: Use Protein A and Ab at 2 µg/mg protein lysate. Immunohistochemistry on Paraffin Embedded Sections: 5-10 µg/ml 30 min RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minuntes followed by cooling at RT for 20 minutes.
Reactivity	All Species
Host	Mouse
Clonality	Monoclonal
Specificity	PY20 recognises Phosphotyrosine, enabling the detection, characterisation and isolation of proteins containing phosphorylated tyrosine residues.  The binding of PY20 to phosphorylated Tyrosines can be inhibited by free Phosphotyrosine and Phenylphosphate, but not by free Phosphate, Phosphoserine or Phosphothreonine.
Formulation	Borate buffered saline pH 8.0 State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets.
Reference Data

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