E. coli Shiga-like Toxin 1 Mouse Monoclonal Antibody [Clone ID: 286-13310]

Specifications

Product Data
Clone Name	286-13310
Applications	ELISA, Neutralize, WB
Recommend Dilution	Toxin Neutralization: Neutralized Vero Cell toxicity of E. coli O157:H7 extracts when used at a 10 µg/ml concentration. Immunoblot: Detected SLT 1 when used at 1 µg/ml concentration. ELISA: Used as capture antibody at 20 µg/ml concentration (100 µl); Immobilizes SLT 1 for detection by an HRP conjugate.
Reactivity	Escherichia coli
Host	Mouse
Clonality	Monoclonal
Immunogen	SLT Toxin from E. coli O157:H7
Specificity	This antibody reacts with alpha, alpha-1 and beta subunits of SLT-1 (STX-1). Also reacts with alpha and alpha-1 subunits of SLT-2 (STX-2).
Formulation	10 mM Phosphate, pH 7.4 containing 0.9% Sodium chloride. State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with 1 ml distilled water
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
Synonyms	Escherichia coli, EHEC, EPEC, ETEC
Reference Data

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