Complement C4A (C4A) Mouse Monoclonal Antibody [Clone ID: 10-12]

Specifications

Product Data
Clone Name	10-12
Applications	ELISA, FC, IHC, WB
Recommend Dilution	Western Blot. Flow Cytometry. ELISA: This antibody binds both C4c and C4 and so is not suitable for all ELISA applications. Immunohistochemistry on Frozen Sections.  Immunohistochemistry on Paraffin Sections: 10 µg/ml. Recommended Postive Control: Kidney sections from post-streptococal glomerulonephritis pastients.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified Human Complement C4c.
Specificity	This antibody recognises complement component 4c (C4c).
Formulation	Borate buffered saline, pH 8.4 State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	complement component 4A (Rodgers blood group)
Database Link	Entrez Gene 720 Human UniProt ID P0C0L4
Background	The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4bs alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. iC4b is further degraded into the inactive fragments C4d and C4c. C4c consists of two a-chain fragments, alpha 4 (13 kDa) and alpha 3 (25 kDa) linked to a beta chain through disulphide bridges and a gamma chain. It is expressed in the blood plasma.
Synonyms	Complement Component 4, CPAMD2, CPAMD3
Reference Data
Protein Pathways	Complement and coagulation cascades, Systemic lupus erythematosus

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.