IL12A Mouse Monoclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, WB
Recommend Dilution	ELISA: In a Sandwich ELISA (assuming 100 µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human IL-12 when used with Biotin anti-Human IL-12 antibody (Cat.-No PP1102B1 or  PP1102B2) as the detection antibody at a concentration of at least 0.25-0.5 µg/ml.  Western Blot: To detect Human IL-12 by Western Blot analysis this antibody can be used at a concentration of 0.50-1.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-12 is 0.5-1.0 ng/lane, under non-reducing conditions.  Immunohistochemistry: This antibody stained PBMC. The primary antibody was incubated at 10.0 µg/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody.  Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	CHO cells derived Highly pure (>98%) 75.0 kDa Recombinant Human IL-12 (Cat.-No PA086).
Specificity	This antibody detects Human Interleukin-12.  Other species not tested.
Formulation	PBS without preservatives State: Azide Free State: Lyophilized (sterile filtered) IgG fraction from Ascites
Reconstitution Method	Restore in sterile water to a concentration of 1.0 mg/ml.
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	interleukin 12A
Database Link	Entrez Gene 3592 Human UniProt ID P29459
Background	IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-γ production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Among its biological activities IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-Gamma producing Th1 cells.
Synonyms	IL-12A, IL-12 subunit p35, NKSF1, CLMF p35
Reference Data
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Allograft rejection, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus

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