ALB Mouse Monoclonal Antibody [Clone ID: 5F9]

Specifications

Product Data
Clone Name	5F9
Applications	ELISA, IHC, IP, WB
Recommend Dilution	ELISA: 1/120,000. Western BLot: 1/2,000-1/10,000. Immunhohistochemistry: 1/1,000-1/5,000. Immunoprecipiation: 1/100.
Reactivity	Bovine
Host	Mouse
Clonality	Monoclonal
Immunogen	Produced after repeated immunizations of balb/c mice with bovine serum albumin.
Specificity	This antibody reacts with Bovine Serum Albumin. It recognizes a 66 kDa band corresponding to Bovine Serum Albumin monomer. No cross reactivity is detected against other serum proteins by immunoblot. No reactivity is detected by ELISA against Human Serum Albumin or Ovalbumin.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 State: Aff - Purified State: Liquid (sterile filtered) purified IgG fraction Stabilizer: None Preservative: 0.01% Sodium Azide
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 280717 Bovine UniProt ID P02769
Background	Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.  Bovine Serum Albumin antibody detects BSA. Serum albumin is the main protein of plasma. It has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. ALB is the major zinc transporter in plasma; it typically binds about 80% of all plasma zinc. BSA antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.
Synonyms	ALB, BSA, HSA, Serum Albumin
Note	Hybridoma: Produced by the fusion between mouse splenocytes and mouse myeloma SP2/0 cells using conventional hybridoma technology.
Reference Data

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