Alpha SNAP (NAPA) Mouse Monoclonal Antibody [Clone ID: 15D4]

Specifications

Product Data
Clone Name	15D4
Applications	ELISA, IP, WB
Recommend Dilution	Western Blot: 0.5-5 ug/ml. Immunoprecipitationon native and denatured samples: 2-10 ug/ml.
Reactivity	Bovine, Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human a-SNAP protein and mouse myeloma cells.
Specificity	This antibody specifically recognizes a-SNAP as a single band of ~36 kDa on western blot of rat kidney, rat brain and MDBK cells and do not recognize b-SNAP.
Formulation	20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50% Glycerol without preservatives. State: Purified State: Liquid purified IgG fraction (> 95% pure).
Concentration	lot specific
Purification	Standard chromatographic techniques.
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	NSF attachment protein alpha
Database Link	Entrez Gene 140673 RatEntrez Gene 8775 Human UniProt ID P54920
Background	SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. a-SNAP (or bSNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas a-SNAP is expressed in all mammalian tissues, b-SNAP is expressed only in brain.
Synonyms	NAPA, SNAP-alpha, SNAPA
Reference Data

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