p53 (TP53) (371-380) Mouse Monoclonal Antibody [Clone ID: HR231]

Specifications

Product Data
Clone Name	HR231
Applications	ELISA, IHC, IP, WB
Recommend Dilution	Western Blot: 1 - 5 µg/ml. ELISA. Immunoprecipitation. Immunohistochemistry. Positive Control: Breast or colon carcinoma cells.
Reactivity	Human, Monkey, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Hybridoma produced by the fusion of splenocytes from immunized with full length human p53 protein and mouse myeloma cells.
Specificity	This antibody reacts to p53.
Formulation	PBS State: Purified State: Liquid purified IgG fraction. Preservative: 0.08% Sodium Azide
Concentration	lot specific
Purification	Affinity chromatography on Protein A/G
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	tumor protein p53
Database Link	Entrez Gene 7157 Human UniProt ID P04637
Background	p53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others.
Synonyms	Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13
Reference Data

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