CD56 (NCAM1) Mouse Monoclonal Antibody [Clone ID: 123C3]

Specifications

Product Data
Clone Name	123C3
Applications	ELISA, FC, IHC
Recommend Dilution	ELISA. Flow Cytometry: Use 10 µl of 1/10-1/50 diluted antibody to label 1x106 cells in 100 µl. Immunohistochemistry on Frozen Tissues. Immunohistochemistry on Paraffin Sections: 1/100-1/200. The antibody requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Small cell lung carcinoma. Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
Specificity	This antibody recognizes Human Neural Cell Adhesion Molecule (NCAM), otherwise known as CD56.  Antibodies produced by clone 123C3 recognizes the 140 and 180kDa isoforms of the CD56 protein.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	neural cell adhesion molecule 1
Database Link	Entrez Gene 4684 Human UniProt ID P13591
Background	The CD56 molecule is a cell surface glycoprotein which is expressed on neuroendocrine cells, natural killer cells and a subset of T cells in the peripheral blood. Three main isoforms of CD56 exist. Neurons express the largest 180kD form, while haemopoietic cells express the 140kD isoform.  In neuronal tissues, CD56 mediates homophilic and heterophilic adhesion and is implicated in neural development. Studies suggest that CD56 is also expressed on thyroid follicular epithelium and may play a role in autoimmune disease of the thyroid. CD56 is expressed in a range of tumours including tumours of the lung, neural derived malignancies and natural killer cell lymphomas.
Synonyms	NCAM-1, N-CAM-1, NCAM
Reference Data

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