B Raf (BRAF) Mouse Monoclonal Antibody [Clone ID: 1H12]

Specifications

Product Data
Clone Name	1H12
Applications	ELISA, IF, IHC, WB
Recommend Dilution	ELISA: 1/10000.  Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000.  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of human BRAF expressed in E. Coli.
Specificity	Recognizes Threonine Protein Kinase BRAF
Formulation	State: Ascites State: Ascitic fluid containing 0.03% Sodium Azide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	87 kDa
Gene Name	B-Raf proto-oncogene, serine/threonine kinase
Database Link	Entrez Gene 673 Human UniProt ID P15056
Background	BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1; RAFB1; B-RAF1; FLJ95109 is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers.
Synonyms	BRAF, BRAF1, RAFB1, p94
Reference Data

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