Pirh2 (RCHY1) Mouse Monoclonal Antibody [Clone ID: 1H10]

Specifications

Product Data
Clone Name	1H10
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA: 1/10000.  Western Blot: 1/500-1/2000.  Immunofluorescence: 1/200-1/1000. Flow Cytometry: 1/200-1/400. Immunohistochemistry on Paraffin Sections: 1/200-1/1000.
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of Human Pirh2 expressed in E. Coli.
Specificity	This antibody recognizes Ring Finger and CHY Zinc Finger Domain Containing 1 (RCHY1).
Formulation	State: Ascites State: Ascitic fluid Preservative: 0.03% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	30 kDa; 60 kDa (homodimer)
Gene Name	ring finger and CHY zinc finger domain containing 1
Database Link	Entrez Gene 25898 Human UniProt ID Q96PM5
Background	Pirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N- and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function.
Synonyms	ARNIP, CHIMP, RNF199, ZNF363
Reference Data

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