NEDD8 Mouse Monoclonal Antibody [Clone ID: 5B8]

Specifications

Product Data
Clone Name	5B8
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA: 1/10000. Western Blot: 1/500-1/2000. Flow Cytometry: 1/200-1/400. Immunofluorescence: 1/200-1/1000.  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of Human NEDD8 expressed in E. Coli.
Specificity	Recognizes NEDD8
Formulation	PBS State: Purified State: Liquid purified antibody Stabilizer: 0.5% protein stabilizer Preservative: 0.05% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	9 kDa
Gene Name	neural precursor cell expressed, developmentally down-regulated 8
Database Link	Entrez Gene 4738 Human UniProt ID Q15843
Background	Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.Tissue specificity: Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
Synonyms	APPBP1; FLJ43224; MGC104393; MGC125896; MGC125897; NAE1; Nedd-8; Neddylin
Reference Data

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