NANOG (1-154) Mouse Monoclonal Antibody [Clone ID: 5A10]

Specifications

Product Data
Clone Name	5A10
Applications	ELISA, FC, IHC, WB
Recommend Dilution	ELISA. Western blot (1/500-1/2,000).  Flow Cytometry. Immunohistochemistry on Paraffin Sections (1/50-1/100).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human Nanog (1-154 aa) purified from E. coli
Specificity	The antibody recognizes NANOG.
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	Nanog homeobox
Database Link	Entrez Gene 79923 Human UniProt ID Q9H9S0
Background	Nanog is a multidomain homeobox transcription factor that functions to maintain the undifferentiated state of pluripotent stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. When Nanog expression is downregulated, cell differentiation can proceed. Proteins that regulate Nanog expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.
Synonyms	FLJ12581; FLJ40451; hNanog
Reference Data

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