Myosin Light Chain 2 (MYL2) (1-166) Mouse Monoclonal Antibody [Clone ID: AT3B2]

Specifications

Product Data
Clone Name	AT3B2
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA. Western blot (1/1,000-1/2,000). Immunohistochemistry on Paraffin Sections (5 µg/ml). This MYL2 antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. Immunocytochemistry / Immunoflourescence. Flow Cytometry.
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human MYL2 (1-166 aa) purified from E. coli
Specificity	The antibody recognizes MYL2 (1-166).
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	myosin light chain 2
Database Link	Entrez Gene 363925 RatEntrez Gene 4633 Human UniProt ID P10916
Background	Myosin light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.
Synonyms	MLC-2v, MLC-2
Reference Data
Protein Pathways	Cardiac muscle contraction, Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction

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