EPCAM Mouse Monoclonal Antibody [Clone ID: ESA214]

Specifications

Product Data
Clone Name	ESA214
Applications	FC, IF, IHC
Recommend Dilution	Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears) For IHC dilute concentared antibody at 1/50-1/100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature. Antigen retriever like pepsin enhances the staining of FFPE tissue. Immunofluorescence: 10-20 µg/ml (1/10-1/20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. Flow Cytometry: 0.2-1.0 µg/0.1 ml (1/200-1/1,000). Recommended Positive Control: Breast carcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Small cell lung carcinoma.
Specificity	This antibody has been used recently to characterize the EpCAM high CD44+ versus EpCAM low/Cd44- indifferent cancer cells. Cellular Localization: Cell membrane.
Formulation	PBS, pH 7.4 containing 1% BSA as stabilizer and 0.05% Sodium Azide as preservative Label: FITC State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Gene Name	epithelial cell adhesion molecule
Database Link	Entrez Gene 4072 Human UniProt ID P16422
Background	Epithelial is a transmembrane glycoprotein (40 kDa) also known as ESA or epithelial cellular adhesion molecule (Ep-CAM). This protein is expressed on basolateral cell surface in most simple epithelia and vast majority of cancers. According to the “Cancer Stem Cells”(CSC) theory, tumors are not viewed as simple monoclonal expression of transformed cells, but rather as complex tissue where abnormal growth is driven by a minority pathological CSC pool that on one hand has acquired tumor related features such as uncontrolled growth and ability to form metasis and on the other hand maintains its inherent capacity to self renew and differentiate into a phenotypic ally heterogeneous, although aberrant progency.
Synonyms	Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2, M4S1, MIC18
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.