Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody [Clone ID: 35betaH11]

CAT#: AM10103PU-N

Cytokeratin 8 (KRT8) mouse monoclonal antibody, clone 35betaH11, Purified



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CNY 5,186.00


货期*
5周

规格
    • 100 ug

Cited in 1 publication.

Product images

Specifications

Product Data
Clone Name 35betaH11
Applications FC, IF, IHC, WB
Recommend Dilution Western blotting. 
Flow Cytometry.
Immunocytochemistry. 
Immunohistochemistry on Frozen Sections

Recommended Dilutions: 1/100–1/500 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/1000 for immunoblotting applications.
Reactivity Human, Zebrafish
Host Mouse
Clonality Monoclonal
Immunogen Cytoskeletal extract of a Human hepatocellular carcinoma cell line (Hep3B).
Specificity 35ßH11 reacts exclusively with Cytokeratin 8 which is present in glandular-type epithelia and most carcinomas derived thereof. It is in general not reactive in non-epithelial tissues and cells.Cytokeratin 8. 
Cellular Localization: Cytoplasmic.
Formulation PBS
State: Purified
State: Liquid purified IgG fraction
Preservative: 0.09% Sodium Azide
Concentration lot specific
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name keratin 8
Background Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are numbered 1 to 20.
The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium.
Cytokeratin subtype expression patterns are used to distinguish between different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only Cytokeratin polypeptides 8 and 18.
Synonyms KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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