Digoxigenin Mouse Monoclonal Antibody [Clone ID: 21H8]

Specifications

Product Data
Clone Name	21H8
Applications	ELISA, IHC, ISH, SB, WB
Recommend Dilution	ELISA: 1/1000-1/10000. Southern Blot: 1/500-1/5000. Western Blot: 1/500-1/5000. In Situ Hybridization: 1/200-1/2000.  Immunohistochemistry on Frozen Sections: 1/200-1/2000.  Immunohistochemistry on Paraffin Sections: 1/200-1/2000.
Host	Mouse
Clonality	Monoclonal
Immunogen	Digoxigenin
Specificity	Reacts with free and bound Digoxigenin.
Formulation	PBS, pH 7.4 Label: HRP State: Lyohilized purified IgG fraction Stabilizer: 50% Glycerol, 1% BSA Preservative: 0.02% Thiomersal
Purification	Affinity Chromatography on Protein A
Conjugation	HRP
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Background	Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
Reference Data

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