GST-Tag Mouse Monoclonal Antibody [Clone ID: 12G8]

CAT#: AM20779PU-N

GST-Tag mouse monoclonal antibody, clone 12G8, Aff - Purified



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CNY 3,674.00


货期*
5周

规格
    • 100 ul

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Specifications

Product Data
Clone Name 12G8
Applications ELISA, WB
Recommend Dilution Western Blot: 1/5000.
Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1 TBS, 0.05% Tween-20 at 4°C with gentle shaking, overnight.
ELISA: 1/2000.
Reactivity All Species, Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Immunogen This monoclonal antibody is produced by immunizing animals with full-length GST.
Specificity This GST-Tag Antibody (12G8) Mouse mAb detects GST and GST fusion proteins.
Formulation State: Aff - Purified
State: Liquid purified Ig fraction
Conjugation Unconjugated
Storage Condition Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Background Plasmid vectors for the expression of coding regions of eukaryotic genes in E.coli are in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. Coli.
Synonyms Glutathione S-transferase Tag, GST26-Tag
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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