Neurofilament H (200 kD) Mouse Monoclonal Antibody [Clone ID: NF-05]

Specifications

Product Data
Clone Name	NF-05
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA (Capture antibody). Western blot: 1-2 μg/ml Positive Control: Lysate of brain homogenate. Immunohistochemistry on Frozen Sections. Has been described to work in Immunohistochemistry on Paraffin Sections.
Reactivity	Human, Porcine, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Pig brain neurofilament protein-enriched fraction after depolymerization of microtubules
Specificity	The antibody NF-05 recognizes a nonphosphorylated epitope of neurofilament heavy protein (NF-H), a 210 kDa intracellular structural protein of Intermediate Filament Proteins family. NF-H is mainly expressed in the central and peripheral nervous system and reproductive system and is biochemically very stable.
Formulation	PBS, pH~7.4 State: Purified State: Liquid purified Ig fraction from Ascites Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Precipitation methods and Size-Exclusion Chromatography (> 95% pure by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Database Link	UniProt ID P12037
Background	Neurofilaments (NFs) are a type of intermediate filaments (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights – neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases.
Synonyms	NEFH,NF-H,KIAA0845, NFH, 200 kDa neurofilament protein, Neurofilament triplet H protein, (Neuronal Marker), heavy polypeptide
Reference Data

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