Basal Membrane Mouse Monoclonal Antibody [Clone ID: PB1]

Specifications

Product Data
Clone Name	PB1
Applications	ELISA, IHC
Recommend Dilution	ELISA. Immunohistochemistry on Frozen Sections: 1/200. Immunohistochemistry on Paraffin Sections: 1/30 (Proteinase K pretreatment for antigen retrieval is recommended).
Reactivity	Human, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Porcine lung extract
Specificity	This antibody recognizes cells of the epithelial basement cell layer in various tissues.
Formulation	PBS, pH 7.2 State: Purified State: Liquid purified Ig fraction Preservative: 0.09% Sodium Azide
Concentration	1.0 mg/ml
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Synonyms	Epithelial Basement cells
Note	Antigen Distribution on Tissue sections: Basal cell layer of various epithelia in different swine organs, e.g. basal cells in the epidermis of the skin, in bronchiolar epithelia of the lung and in cells of the macula densa in the kidney. Protocol: Staining Procedure For Frozen And Paraffin-EmbeddedTissue Sections  Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse lgM) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer’s hemalum.  Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature 1. Deparaffinize and rehydrate tissue section 2. Incubate the tissue section with proteinase K for 7min 3. Block endogenous peroxidase 4. Wash in PBS 5. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 6. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 7. Wash in PBS 8. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgM) for 1h in a humid chamber 9. Wash in PBS 10. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 11. Wash in PBS 12. Counterstain with Mayer’s hemalum
Reference Data

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