CHRM2 (168-192) Mouse Monoclonal Antibody [Clone ID: B8E5]

CAT#: AM26189PU-N

CHRM2 (168-192) mouse monoclonal antibody, clone B8E5, Purified



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CNY 6,281.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name B8E5
Applications ELISA, FN, IF, IHC, WB
Recommend Dilution Flow Cytometry.
Functional Assays (Agonist-like antibody).
Immunoassay.
Immunofluorescence.
Western blot (Non-Reduced Conditions, specific bands of ~55, 63 and 65 kDa).
The typical starting working dilution is 1/50.
Reactivity Guinea Pig, Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Immunogen Human second extracellular loop residues 168-192
Specificity The monoclonal antibody B8E5 recognizes Human muscarinic acetylcholine receptor M2 (M2 receptor), a G protein-coupled cardiovascular receptor of ~55 kDa.
Monoclonal antibody B8E5 inhibits the ß-adrenergic L-type Ca2+ currents through activation of the muscarinic acetylcholine receptor M2. It suggests that the antibody acts not via the classical pathway of decreasing cAMP, but rather by increasing cGMP.
Monoclonal antibody B8E5 acts by functional dimerization of the receptor resulting in stabilization of the constitutive active receptor dimers and paradoxically induces a small decrease in carbachol affinity for the M2 receptor. It recognizes the pentapeptide VRTVE (aa 168-172) corresponding to the N-terminal part of the second extracellular loop of the Human M2 receptor.
Formulation PBS
State: Purified
State: Liquid 0.2 µm filtered Ig fraction
Stabilizer: 0.1% BSA
Concentration lot specific
Purification Protein G Chromatography
Conjugation Unconjugated
Storage Condition

Store undiluted at 2-8°C.

Gene Name cholinergic receptor muscarinic 2
Background This receptor is an integral membrane protein consisting of seven membrane spanning a-helices linked together by extra- and intracellular loops that form a pharmacophore pocket. Autoantibodies directed against cardiovascular G protein-coupled receptors functionally interfering with the target have been described in several cardiovascular diseases. The M2 receptor is the predominant sybtype of muscarinic receptors present in the heart of mammalian species. The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
Synonyms CHRM2, mAChR M2, mAChR-M2
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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