Mbl2 Rat Monoclonal Antibody [Clone ID: 14D12]

Specifications

Product Data
Clone Name	14D12
Applications	ELISA, IF, IHC, WB
Recommend Dilution	Immunohistochemistry on frozen sections (4): Cryostat sections (7µm) of mouse tissue were acetone fixed and stained o/n at 4°C in TBS/2% goat serum. (Ref.4). The typical starting working dilution is 1:10. It is recommended that solutions with a calcium concentration of 1 mM are used (14D12 is a calcium-dependent antibody). Immunoassays (1). Immunoflourescence (3,5): Hyphae of C. albicans were fixed on poly-l-lysin coated slides with ice-cold aceton. Slides were incubated for 1h at RT with 14D12. (Ref.3). Western blot (1,2): On a 12% non-reducing SDS-PAGE a prominent band of ca. 50Kda is detected. Under reducing conditions a 26 KDa band is detected. (Ref.2). The typical starting working dilution is 1:10. It is recommended that solutions with a calcium concentration of 1 mM are used (14D12 is a calcium-dependent antibody). Positive control: Mouse Serum and kidney tissue.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Purified MBL-C
Specificity	This antibody detects MBL-C.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin Preservative: 0.02% sodium azide
Concentration	lot specific
Purification	Protein G
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C.
Gene Name	mannose-binding lectin (protein C) 2
Database Link	Entrez Gene 17195 Mouse UniProt ID P41317
Background	Mannose binding lectin (MBL), also called mannose- or mannan-binding protein (MBP), is a member of the group of collectins. MBL is an important pattern-recognition receptor in the innate immune system. The protein mediates innate immune responses, such as activation of the complement lectin pathway and phagocytosis, to help fight infections. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. Binding of MBL leads to the activation of MBL-associated serine proteases (MASP's). Activated MASP-2 cleaves C4 and C2 in a similar way as C1s do for the classical pathway (CP) leading to the formation of C4b2a, cleavage of the classical pathway convertase C3, and eventually complement activation up to the formation of the membrane attack complex. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. MBL is predominantly synthesized by hepatocytes and has been isolated from the liver or serum of several vertebrate species. Only one form of human MBL has been characterized, while two forms are found in rhesus monkeys, rabbits, rats and mice. The mouse forms are known as MBL-A and MBL-C. The MBL-C concentrations in serum are about 6-fold compared to that of MBL-A. MBL-A, but not MBL-C, was found to be an acute phase protein in casein and LPS-injection models. MBL-C exists in higher oligomeric forms than MBL-A.
Synonyms	MBP, Mannose-binding protein C, MBP-C, MBP1, Mannan-binding protein, Mannose-binding lectin, MBL2, MBL
Reference Data

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