EPOR (Cytoplasm. Dom.) Rat Monoclonal Antibody [Clone ID: BCO-3H2]

Specifications

Product Data
Clone Name	BCO-3H2
Applications	ELISA, FC, WB
Recommend Dilution	Flow Cytometry only on permeabilised cells: 1.2 μg/106 cells. ELISA: 1/200-1/400. Cell based ELISA only on permeabilised cells: 1/200-1/400. Western blot: See ref.5. Immunohistochemistry: See ref.5.
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	Peptide immunisation from the cytoplasmic domain of human Epo-R
Specificity	This antibody detects Human Epo-R. Other species not tested.
Formulation	Phosphate buffered saline, pH 7.2 State: Purified State: Liquid Ig fraction
Purification	Protein G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	erythropoietin receptor
Database Link	Entrez Gene 2057 Human UniProt ID P19235
Background	Erythropoietin receptor (Epo-R) belongs to the cytokine receptor family and is a 507 amino acid type I transmembrane protein. EpoR pre-exists as dimers which changes the homodimerized state after binding of its 34 kDa ligand erythropoietin (Epo) (1,2). Erythropoietin is the primary reg ulator of erythropoiesis, and promotes the survival, proliferation, and differentiation of erythroid progenitor cells. Both, Epo and Epo-R are essential for the production of red blood cells due to Epo exerts its function through the E po receptor (3). The Epo-R is also expressed in many organs outside the bone marrow, suggesting that Epo is a pleiotropic anti-apoptotic factor. Signaling pathways have been shown to influence numerous cellular functions in normal and tumor cells, including proliferation, apoptosis, and drug resistance (4). Development and specificity testing of the antibody are described in detail elsewhere (5).
Synonyms	EPOR, EPO-R
Reference Data

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