Cd27 Hamster Monoclonal Antibody [Clone ID: LG.3A10]

Specifications

Product Data
Clone Name	LG.3A10
Applications	FC, FN, IHC
Recommend Dilution	ELISA: In combination with LG.8A6, it is possible to develop a double determinant ELISA to detect soluble Mouse CD27. Flow Cytometry: Functional Studies (stimulating). Immunohistochemistry on Frozen Sections.  Immunohistochemistry on Paraffin Sections: This clone (LG.3A10) has been reported to work on Paraffin tissue sections.
Reactivity	Mouse
Host	Hamster
Clonality	Monoclonal
Immunogen	MCD27 tag expressing clones AR0524 and AR0530 from Armenian Hamster spleen. Fusion Partner: SP2/0 Murine Myeloma Partner.
Specificity	Recognizes Mouse CD27 receptor.
Formulation	PBS containing 0.02% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction from Ascites
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD27 antigen
Database Link	Entrez Gene 21940 Mouse UniProt ID P41272
Background	CD27 is part of the Tumor Necrosis Factor (TNF) family of receptors and is lymphocyte specific. Murine CD27 is expressed on the great majority of both and T lymphocytes, on a small population of peripheral B cells and on a very small subset of B220+ cells in the bone marrow. Murine CD27 is found on all thymocytes except a subset of CD4-CD8- precursors. There are two known basic functions of CD27 signaling. First, the binding of CD27 to CD70, a protein which is also expressed in T and B cells, provides costimulatory signals in lymphocyte proliferation and immunoglobulin production. Second, the association of CD27 with intracellular Siva, a proapoptotic protein, results in the induction of apoptotic events.
Synonyms	TNFRSF7, CD27L receptor, T14
Reference Data

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