TNF alpha (TNF) Mouse Monoclonal Antibody [Clone ID: T1]

Specifications

Product Data
Clone Name	T1
Applications	FN, IP, WB
Recommend Dilution	Western blotting: Use 1/10 as a starting dilution.  Flow Cytometry:  Use 1/10 as a starting dilution.  Immuno Assays.  Immunoprecipitation.  Inhibition of the Biological Activity:  For Neutralization of biological activity in vitro dilutions have to be made according to the amounts of TNF-alpha to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	The antibody reacts with free soluble (17 kDa) and membrane (26 kDa) Human TNF-alpha. The antibody inhibits the biological activity of both forms. It does not react with receptor bound TNF-alpha. It can be a useful tool to discriminate between the membrane form of TNF expressed on producer cells and the proteolytically cleaved, soluble TNF-apha bound to its cognate cell membrane receptors (TNF-RI and TNF-RII). For this purpose we recommend to use this antibody in combination with the anti-TNF-alpha antibody Cat.-No AM32018 clone T3, which recognizes soluble, membrane and receptor bound TNF-alpha
Formulation	PBS State: Purified State: Liquid (0.2 μm filtered) Ig fraction Stabilizer: 0.1% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	tumor necrosis factor
Database Link	Entrez Gene 7124 Human UniProt ID P01375
Background	Tumor necrosis factor (TNF, cachexin or cachectin and formally known as tumor necrosis factor alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis, and viral replication. TNF's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases, as well as cancer.
Synonyms	TNF, TNF-a, TNFA, TNFSF2, Cachectin
Reference Data

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