TNF alpha (TNF) Mouse Monoclonal Antibody [Clone ID: 4H31]

Specifications

Product Data
Clone Name	4H31
Applications	ELISA, FN, IHC, IP, WB
Recommend Dilution	Western blotting.  Flow Cytometry.   Immuno Assays.  Immunoprecipitation.  Immunohistochemistry on Frozen Sections of inflamed tissues. Inhibition of the Biological Activity: It has been tested in Cytotoxicity assays as well as in assays with activation of endothelial cells and found to be a powerful antibody. It is can be used to discriminated between TNF-alpha and TNF-beta or lymphotoxin.  Dilutions have to be made according to the amount of TNF-alpha to be inactivated.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Specificity	The antibody reacts with Human native and recombinant TNF-alpha as assessed by ELISA. The antibody inhibits the biological activity of Human native and recombinant TNF-alpha as determined with L929 cells in a Cytotoxicity Assay. The antibody crossreacts with Rhesus and Cynomolgus natural TNF-α and lacks crossreactivity with Human lymphotoxin.
Formulation	PBS State: Purified State: Liquid (0.2 μm filtered) purified Ig fraction Stabilizer: 0.1% BSA
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	tumor necrosis factor
Database Link	Entrez Gene 7124 Human UniProt ID P01375
Background	Tumor necrosis factor (TNF, cachexin or cachectin and formally known as tumor necrosis factor alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis, and viral replication. TNF's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases, as well as cancer.
Synonyms	TNF, TNF-a, TNFA, TNFSF2, Cachectin
Reference Data

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