EMA (MUC1) Mouse Monoclonal Antibody [Clone ID: GP1.4]

Specifications

Product Data
Clone Name	GP1.4
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA (For coating, order Ab without BSA). Western Blot: 0.5-1 µg/ml.  Flow Cytometry: 0.5-1 µg/106 cells.  Immunofluorescence: 1-2 µg/ml.  Immunoprecipitation: 1-2 µg/500 µg protein lysate. Immunohistochemistry on Frozen and Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. No special pretreatment is required for staining of formalin/paraffin tissues. Recommended Positive Control: MCF-7 or MDA-231 cells, Breast or Colon carcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human milk fat globule membranes.
Specificity	This GP1.4 Monoclonal antibody (Cat.-No AM32842PU) is specific to epithelial membrane antigen (EMA), also called Mucin-1 (MUC-1), CA15-3, CA27-29, CD227, DF3, H23AG, KL-6, MAM6, PEM, PEMT, and PUM. In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. This GP1.4 Monoclonal antibody (Cat.-No AM32842PU) reacts with the DTRP epitope in the tandem repeats. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues without any special pretreatment. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. Cellular Localization: Cell membrane and cell surface.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	265-400 kDa
Gene Name	mucin 1, cell surface associated
Database Link	Entrez Gene 4582 Human UniProt ID P15941
Background	MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.
Synonyms	MUC-1, PEMT, Episialin, EMA, H23AG, PUM, DF3, CA 15-3
Reference Data

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