EGFR Mouse Monoclonal Antibody [Clone ID: DP-4A1]

Specifications

Product Data
Clone Name	DP-4A1
Applications	ELISA, FC, WB
Recommend Dilution	Flow Cytometry: 1.2 µg/106 cells. Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	cDNA encoding Human ErbB-1
Specificity	Recognizes Epidermal growth factor receptor (EGFR; ErbB-1). Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells.
Formulation	PBS, pH 7.2 State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	epidermal growth factor receptor
Database Link	Entrez Gene 1956 Human UniProt ID P00533
Background	Epidermal growth factor receptor (EGFR; ErbB-1) is a 170-kDa transmembrane glycoprotein encoded by the HER1 proto-oncogene. It belongs to the EGFR family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her3 (ErbB-3) and Her4 (ErbB-4). ErbB-1 is expressed on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). Growth factor binding to the extracellular domain of ErbB-1 induces formation of homoand heterodimers with another member of the ErbB receptor family. The EGFR signaling pathway is one of the most important pathways that regulate growth, survival, proliferation and differentiation in mammalian cells, while over-expression of ErbB-1 have been associated with a number of cancers, including lung cancer and glioblastoma (2,3).
Synonyms	Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Reference Data

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