ACTC1 Mouse Monoclonal Antibody [Clone ID: 22D3]

Specifications

Product Data
Clone Name	22D3
Applications	ELISA, IF, IHC, WB
Recommend Dilution	ELISA.  Flow Cytometry.  Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Sections.  Recommended Dilutions: 1/100-1/1000 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/1000-1/5000 for immunoblotting applications.
Reactivity	Human, Porcine, Rabbit, Rat, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide comprising the N-terminal nonapeptide of α-Cardiac Actin with an acetylated N-terminus coupled to keyhole limpet hemocyanin through the cysteine residue.
Specificity	This 22D3 Monoclonal antibody is highly specific for Alpha-Cardiac Actin, and does not cross react with other Actin isoforms.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles.
Gene Name	actin, alpha, cardiac muscle 1
Database Link	Entrez Gene 70 Human UniProt ID P68032
Background	Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus,  but the sequence of each of these actins is highly conserved in higher vertebrates. Cardiac α-actin, which is the main actin isoform in the adult heart, has also been shown to be the predominant form in early muscle development. In later development the expression of α-cardiac actin is down regulated and α-skeletal actin becomes the dominant isoform in the adult skeletal muscle.
Synonyms	ACTC; ASD5; CMD1R; CMH11
Reference Data

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