Integrin beta 4 (ITGB4) Mouse Monoclonal Antibody [Clone ID: 58XB4]

Specifications

Product Data
Clone Name	58XB4
Applications	ELISA, FC, IF, IHC, IP, WB
Recommend Dilution	ELISA.  Western blotting. Immunofluorescence.  Flow Cytometry.  Immunoprecipitation.  Imunocytochemistry of paraformaldehyde fixed cells. Immunohistochemistry on Frozen Sections.  See Reference 1 for Protocols.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Immunization of Balb/c mice with Integrin β4. The immunogen used to raise this antibody was the full length CD104 protein.
Specificity	Binds specifically to integrin β4 chain(CD104) or β4 integrin, that associates with integrin α6 (CD49f) forms α6/β4 (CD49f/CD104) heterodimer. CD104 is expressed on epithelial cells (especially on the proliferative basal layer epithelial cells in skin), endothelial cells, Schwann cells, certain tumor cells and a subset of pre-T cells.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles.
Gene Name	integrin subunit beta 4
Database Link	Entrez Gene 3691 Human UniProt ID P16144
Background	Integrins are heterodimers composed of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. Integrin b4 (CD104)  associates with integrin α6 (CD49f) forming the α6/β4 (CD49f/CD104) heterodimer. CD104 is expressed on epithelial cells (especially on the proliferative basal layer epithelial cells in skin), endothelial cells, Schwann cells, certain tumor cells and a subset of pre-T cells. CD49f/CD104 is an adhesion receptor for laminins (especially laminin 5) and keratin filaments and is involved in the regulation of hemidesmosome formation and of cell proliferation and activation. CD104 is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Synonyms	ITGB4, GP150
Reference Data

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