CA 19-9 Mouse Monoclonal Antibody [Clone ID: 121SLE]

Specifications

Product Data
Clone Name	121SLE
Applications	FC, IF, IHC, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Western Blot: 0.5-1.0 µg/ml. Flow Cytometry: 0.5-1.0 µg/106 cells. Immunofluorescence: 0.5-1.0 µg/ml. Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1.0 µg/ml for 30 minutes at RT.  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Stomach or Colon Carcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Precipitin lines obtained after immuno-diffusion using MAb 116-NS-19-9 and mucins isolated from an ovarian cyst of a Lewis A+B- patient (0Le).
Specificity	This 121SLE antibody is specific to CA19-9, which can predict recurrence of disease prior to radiographic treatment. Cellular Localization: Cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Predicted Protein Size	> 400 kDa
Background	CA19-9, a carbohydrate epitope expressed on a high MW (> 400kDa) mucin glycoprotein, is a sialyl Lewisa structure which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lewisa and/or Lewisb blood group antigens. In normal tissues, sialyl Lewisa antigen is present in ductal epithelium of the breast, kidney, salivary gland, and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas, including adenocarcinomas of the stomach, intestine, and pancreas. Preoperative elevated CA19-9 levels in patients with stage I pancreatic carcinoma decrease to normal values following surgery. When used serially, CA19-9 can predict recurrence of disease prior to radiographic or clinical findings.
Synonyms	CA19-9, Sialosyl Lewis a, Sialyl Lewis a
Reference Data

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