Phosphotyrosine Mouse Monoclonal Antibody [Clone ID: SPM102]

Specifications

Product Data
Clone Name	SPM102
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for Coating. Western Blot: 0.5-1 µg/ml. Flow Cytometry: 0.5-1 µg/106 cells.   Immunofluorescence: 1-2 µg/ml.  Immunoprecipitation: 1-2 µg/500 µg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 1-2 µg/ml for 30 minutes at RT.  No special pretreatment is required for staining of formalin/paraffin tissues.  Recommended Positive Control: MCF-7, MDA-231, T47-D cells or breast carcinoma.
Reactivity	All Species
Host	Mouse
Clonality	Monoclonal
Immunogen	Phosphotyrosine conjugated to KLH
Specificity	Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This Monoclonal antibody shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues. Cellular Localization: Depends upon the location of phosphorylated target.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Predicted Protein Size	Depends upon the phosphorylated target
Background	Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes.  Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases.  The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates.  Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation.
Reference Data

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