Biotin Mouse Monoclonal Antibody [Clone ID: SPM375]

Specifications

Product Data
Clone Name	SPM375
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA.  Western Blotting. Confocal Microscopy.  Flow Cytometry: 0.5-1 µg/106 cells.  In situ Nucleic Acid Hybridization.  Immunofluorescence: 0.5-1 µg/ml.  Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 µg/ml for 30 minutes at RT.  No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues. Recommended Positive Control: Biotinylated proteins in solution or on tissues.
Reactivity	All Species
Host	Mouse
Clonality	Monoclonal
Immunogen	Biotinylated Sheep immunoglobulin.
Specificity	It recognizes both the free and protein-conjugated (either soluble or cell bound) form of Biotin.  This Monoclonal Antibody is highly specific to Biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for Biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-Biotin antibody may be substituted to decrease this noise. Cellular Localization: Depends on the target.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Predicted Protein Size	244 kDa
Synonyms	Vitamin B7, Vitamin H
Reference Data

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