Bromodeoxyuridine / BrDU Mouse Monoclonal Antibody [Clone ID: SPM166]

Specifications

Product Data
Clone Name	SPM166
Applications	FC, IF, IHC
Recommend Dilution	Flow Cytometry: 0.5-1 µg/106 cells.  Immunofluorescence: 0.5-1 µg/ml.  Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.  For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37°C. Recommended Positive Control: Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.
Reactivity	All Species
Host	Mouse
Clonality	Monoclonal
Immunogen	Bromodeoxyuridine (BrdU) conjugated to KLH
Specificity	It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.  Cellular Localization: Nuclear.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Predicted Protein Size	307 kDa
Background	Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells.
Reference Data

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