Il6 Rat Monoclonal Antibody [Clone ID: MP5-20F3]

Specifications

Product Data
Clone Name	MP5-20F3
Applications	FC, IF, IHC, IP, Neutralize, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating.  Western Blotting: 0.5-1 µg/ml. Neutralization Studies: Use Azide free Antibody (Reported in Litterature). Flow Cytometry: 0.5-1 µg/million cells.  Immunofluorescence: 0.5-1 µg/ml. Immunoprecipitation: 0.5-1 µg/500 µg protein lysate.  Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT (Reported in Litterature). Positive Control: Mouse Heart.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Recombinant Mouse IL-6 protein.
Specificity	This antibody is specific for Mouse Interleukin 6 (IL-6). Clone MP5-20F3 may be used to neutralise IL-6 bioactivity in a Functional Assay (Abrams et al. 1992 and Kambayashi et al. 1995). Cellular Localization: Cytoplasmic and extracellular (secreted).  Negative Species: Human, Rat.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	21 kDa
Gene Name	interleukin 6
Database Link	Entrez Gene 16193 Mouse UniProt ID P08505
Background	IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B-cells and T-cells. IL-6 plays a role in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by T-cells, B-cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes.
Synonyms	IL-6, Interferon beta-2, IFNB2, B-cell stimulatory factor 2, BSF-2, CDF
Reference Data

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