CD45 (PTPRC) (CD45RB) Mouse Monoclonal Antibody [Clone ID: BRA-11; same as BRA-11G]

Specifications

Product Data
Clone Name	BRA-11; same as BRA-11G
Applications	IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 µg/million cells. Immunofluorescence: 0.5-1 µg/ml. Western Blotting: 0.5-1.0 µg/ml. Immunoprecipitation: 0.5-1 µg/500 µg protein lysate. Immunohistochemistry on Frozen and  Formalin-Fixed Paraffin Sections: 0.5-1.0 µg/ml for 30 minutes at RT. (taining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Ramos, U-698, or GA-10 cells, Tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Non-T, non-B CALLA positive ALL cell line REH (Leucocyte Workshop IV and V).
Specificity	Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. Cellular Localization: Cell surface and cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	180-220 kDa
Gene Name	protein tyrosine phosphatase, receptor type C
Database Link	Entrez Gene 5788 Human UniProt ID P08575
Background	CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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