WNT3A Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Human WNT-3a by Direct ELISA (using 100 μl/well antibody solution) a concentration of ~1.0 μg/ml is required. This biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human WNT-3a. Sandwich ELISA: To detect Human WNT-3a by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human WNT-3a (Cat.-No AP00591PU-N or AP00591PU-S) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human WNT-3a. Western Blot: To detect Human WNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	E.Coli derived recombinant Human WNT-3a
Specificity	This antibody detects Human WNT-3a. Other species not tested.
Formulation	PBS, pH 7.2 Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month  or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	Wnt family member 3A
Database Link	Entrez Gene 89780 Human UniProt ID P56704
Synonyms	Protein Wnt-3a
Note	Centrifuge vial prior to opening.
Reference Data

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